ENCBS495ZOS / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP539 (unc-119(tm4063) III; wgIs539) hermaphrodite L1 larva 6 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans nhr-85
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to NHR-85
Life stage
L1 larva
Age
Unknown
Synchronization timepoint
L1 larva starved after bleaching + 6.0 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:NHR-85_GFP_OP539_L1_INPUT

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM287GCKinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO860KCO
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063) III; wgIs539
Sex
hermaphrodite
Strain name
OP539
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale