1. Biosamples
  2. cell line
  3. Homo sapiens
  4. HepG2

ENCBS464DPO / cell line

Summary

Status
released
Term name
HepG2
Summary
Homo sapiens HepG2 cell line genetically modified (insertion) using CRISPR targeting H. sapiens MBNL3
Separated from biosample
ENCBS475JUN

Attribution

ENCODE4 project
Lab
Michael Snyder, Stanford
Award PI
Michael Snyder, Stanford
Submitted by
Tao Wang
Source
ATCC
Project
ENCODE
Aliases
michael-snyder:pB-14105, michael-snyder:pB-eGFP-MBNL3 HepG2 Batch 2 Rep4

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM756CWAinsertiontaggingCRISPR

Donor information

Status
released
Accession
ENCDO000AAC
Aliases
encode:donor of HepG2, bradley-bernstein:Donor of HepG2
Species
Homo sapiens
Life stage
child
Age
15 years
Sex
male
Health status
hepatocellular carcinoma
Ethnicity
European
External resources
References

Documents

immunoblot
Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…
Attachment from submitter
Attachment from submitters
69 IP_HepG2_MBNL3(GFP)_Rep2.jpg
Caption
Immunoprecipitation was performed on nuclear extracts from biosample ENCBS464DPO (HepG2 eGFP-MBNL3 replicate 1) cells using anti-eGFP antibody.The image shows western blot analysis of input (lane 1), immunoprecipitate (lane 2), and mock immunoprecipitate using IgG (lane 3). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD). The target molecular weight is 67.0 kD as indicated with an arrow.
Submitted by
Tao Wang
Lab
Michael Snyder, Stanford