ENCBS463HXN / cell line

Summary

Status: released
Term name: A549
Term ID: EFO:0001086
Summary: Homo sapiens A549 cell line genetically modified (insertion) using CRISPR targeting H. sapiens PRDM1
Separated from biosample: ENCBS830VTV
Parent of biosamples: ENCBS223AKZ, ENCBS810DVN

Attribution

Lab: Michael Snyder, Stanford
Award PI: Michael Snyder, Stanford
Submitted by: Nathaniel Watson
Source: Michael Snyder
Project: ENCODE
External resources: Cellosaurus:CVCL_0023
Aliases: michael-snyder:pB-11140, michael-snyder:pB-eGFP-PRDM1 A549

Genetic modifications

Accession	Category	Purpose	Method	Nucleic acid delivery method	Site
ENCGM553STI	insertion	tagging	CRISPR		Target PRDM1-human

Donor information

Status: released
Accession: ENCDO000AAZ
Aliases: encode:donor of A549, bradley-bernstein:Donor of A549 cells, john-stamatoyannopoulos:ATCC_A549
Species: Homo sapiens
Life stage: adult
Age: 58 years
Sex: male
Ethnicity: European
External resources: GEO:SAMN05733878
References: PMID:4357758

Documents

Description: Cell growth protocol for A549 cells (version 1).

Attachment PDF Icon

ENCODE4_Snyder_CellGrowth_A549_v1.pdf

Submitted by: Nathaniel Watson
Lab: Michael Snyder, Stanford
Grant: U54HG006996

Description excerpt: A549 transfection protocol - Version 1 (Replicates split right after…

Attachment PDF Icon

ENCODE4_Snyder_CellTransfection_A549_v1.pdf

Description: A549 transfection protocol - Version 1 (Replicates split right after transfection, 500 µg/ml G418)
Submitted by: Nathaniel Watson
Lab: Michael Snyder, Stanford
Grant: U54HG006996