ENCBS445AAA / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line stably expressing C-terminal eGFP-tagged NR4A1
Description
K562 cell line stably expressing a C-terminal LAP-tag containing eGFP, fused to NR4A1
Culture harvest date
2012-12-13
Passage number
5

Attribution

ENCODE3 project
Lab
Kevin White, UChicago
Award PI
Michael Snyder, Stanford
Submitted by
Jay Rehm
Project
ENCODE
Aliases
kevin-white:NR4A1_GFP_2
Submitter comment
Biorep2

Genetic modifications

Accession
Category
Purpose
Method
Site
ENCGM696LPOinsertiontaggingstable transfection

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
External resources
References

Documents

immunoblot
Caption excerpt: Western blot analysis of nuclear lysates prepared from a K562 stable cell line…
Attachment from submitter
Caption
Western blot analysis of nuclear lysates prepared from a K562 stable cell line expressin eGFP-NR4A1 using the Roche anti-GFP 11814450001 antibody. The expected MW is 97.99, 99.53kDa.
Submitted by
Mark Murphy
Lab
Kevin White, UChicago
transfection protocol
Description: UChicago IGSB ENCODE K562 Transfection Protocol March 5, 2013
Submitted by
Jay Rehm
Lab
Kevin White, UChicago
immunoblot
Caption excerpt: Western blot analysis of nuclear lysates prepared from a K562 stable cell line…
Attachment from submitter
Caption
Western blot analysis of nuclear lysates prepared from a K562 stable cell line expressing eGFP-NR4A1 using the Roche anti-GFP 11814450001 antibody. The expected MW is 97.99, 99.53kDa.
Submitted by
Jay Rehm
Lab
Kevin White, UChicago
FACs analysis
Caption excerpt: eGFP-NR4A1 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby…
Attachment from submitter
Caption
eGFP-NR4A1 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP+ fraction quantified for their GFP and nuclear signal colocalization.
Submitted by
Mark Murphy
Lab
Kevin White, UChicago
FACs analysis
Caption excerpt: eGFP-NR4A1 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby…
Attachment from submitter
Caption
eGFP-NR4A1 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP++ fraction quantified for their GFP and nuclear signal colocalization.
Submitted by
Mark Murphy
Lab
Kevin White, UChicago
FACs analysis
Caption excerpt: eGFP-NR4A1 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby…
Attachment from submitter
Caption
eGFP-NR4A1 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP+ fraction quantified for their GFP and nuclear signal colocalization.
Submitted by
Mark Murphy
Lab
Kevin White, UChicago