ENCBS439BBX / cell line

Status: released
Term name: A549
Term ID: EFO:0001086
Summary: Homo sapiens A549 cell line genetically modified (insertion) using CRISPR targeting H. sapiens NR5A2
Culture harvest date: 2018-05-07
Separated from biosample: ENCBS723DBL

Accession	Category	Purpose	Method	Nucleic acid delivery method	Site
ENCGM623TXY	insertion	tagging	CRISPR		Target NR5A2-human

Status: released
Accession: ENCDO000AAZ
Aliases: encode:donor of A549, bradley-bernstein:Donor of A549 cells, john-stamatoyannopoulos:ATCC_A549
Species: Homo sapiens
Life stage: adult
Age: 58 years
Sex: male
Ethnicity: European
External resources: GEO:SAMN05733878
References: PMID:4357758

Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…

Caption: Immunoprecipitation was performed on nuclear extracts from biosample ENCBS439BBX (A549 eGFP-NR5A2 replicate 2) cells using anti-eGFP antibody. The image shows western blot analysis of input (lane 1), immunoprecipitate (lane 2), and mock immunoprecipitate using IgG (lane 3). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD). The target molecular weight is 90.0 kD as indicated with an arrow.
Submitted by: Nathaniel Watson
Lab: Michael Snyder, Stanford
Grant: UM1HG009442
Submitter comment: Band is within 20% of expected target size (90-20%=72).
Documents: michael-snyder:E4_P3_IP-WB_v2

Description: Cell growth protocol for A549 cells (version 1).

Description excerpt: A549 transfection protocol - Version 1 (Replicates split right after…

Description: A549 transfection protocol - Version 1 (Replicates split right after transfection, 500 µg/ml G418)
Submitted by: Nathaniel Watson
Lab: Michael Snyder, Stanford
Grant: U54HG006996