ENCBS435FHS / in vitro differentiated cells
Summary
- Status
- released
- Term name
- neuroepithelial stem cell
- Term ID
- CL:0002259
- Summary
- Homo sapiens female embryo (5 days) neuroepithelial stem in vitro differentiated cells stably expressing C-terminal eGFP-tagged HES5 originated from H9
- Description
- Neuroepithelial cells were collected at day 12 of differentiation (see protocol)
- Culture start date
- 2011-03-17
- Culture harvest date
- 2011-03-30
- Originated from biosample
- ENCBS769EPD
Attribution
ENCODE3 project
- Lab
- Bradley Bernstein, Broad
- Award PI
- Bradley Bernstein, Broad
- Submitted by
- Noam Shoresh
- Source
- Alex Meissner
- Project
- ENCODE
- External resources
- Aliases
- bradley-bernstein:BioSam 1858
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM252BLF | insertion | tagging | stable transfection |
|
Donor information
- Status
- released
- Accession
- ENCDO222AAA
- Aliases
- encode:donor of H9, roadmap-epigenomics:113, john-stamatoyannopoulos:H9 FUCCI, bradley-bernstein:Donor of H9 cells
- Species
- Homo sapiens
- Life stage
- embryonic
- Age
- 5 days
- Sex
- female
- Health status
- healthy
- External resources
Documents
general protocol
Description: H9_derived_differentiation_protocol.pdf
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
certificate of analysis
Description: WiCell: Certificate of Analysis - Amended for H9
- Submitted by
- Kathrina Onate
- Lab
- J. Michael Cherry, Stanford
- Grant
- U24HG009397