ENCBS410VSB / cell line

Summary

Status
released
Term name
A549
Summary
Homo sapiens A549 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (pgRNA) 0 days after the sample was treated with 2 μg/mL Puromycin for 2 days, 0.2 μg/mL Puromycin for 2 days
Culture start date
2019-05-25
Culture harvest date
2019-07-10

Attribution

ENCODE4 project
Lab
Bing Ren, UCSD
Award PI
Yin Shen, UCSF
Submitted by
Bin Li
Source
ATCC
Project
ENCODE
External resources
Aliases
bing-ren:BC19B_A549_D0_B1_BC102

Treatment details

Treatment
2 μg/mL Puromycin (CHEBI:17939) for 2 days
Type
chemical
Purpose
selection
Treatment
0.2 μg/mL Puromycin (CHEBI:17939) for 2 days
Type
chemical
Purpose
selection

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM554RTSinsertionexpressiontransduction
ENCGM353IMQCRISPRicharacterizationCRISPR

Donor information

Status
released
Accession
ENCDO000AAZ
Aliases
encode:donor of A549, bradley-bernstein:Donor of A549 cells, john-stamatoyannopoulos:ATCC_A549
Species
Homo sapiens
Life stage
adult
Age
58 years
Sex
male
Ethnicity
European
External resources
References