ENCBS407QJE / cell line

Summary

Status
released
Term name
A549
Summary
Homo sapiens A549 cell line genetically modified (episome) using transient transfection treated with 100 nM dexamethasone (agonist) for 12 hours

Attribution

Genomics of Gene Regulation Project
Lab
Tim Reddy, Duke
Award PI
Tim Reddy, Duke
Submitted by
Alejandro Barrera
Source
ATCC
Project
GGR
External resources
Aliases
tim-reddy:ggr-starrseq-output-dex-12h-rep1-biosample

Treatment details

Treatment
100 nM dexamethasone (CHEBI:41879) for 12 hours
Type
chemical (agonist)
Purpose
perturbation

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM086KMSepisomecharacterizationtransient transfection

Donor information

Status
released
Accession
ENCDO000AAZ
Aliases
encode:donor of A549, bradley-bernstein:Donor of A549 cells, john-stamatoyannopoulos:ATCC_A549
Species
Homo sapiens
Life stage
adult
Age
58 years
Sex
male
Ethnicity
European
External resources
References

Documents

treatment protocol
Description: ChIP_DEX_treatment_protocol
Submitted by
Linda Hong
Lab
Tim Reddy, Duke
treatment protocol
Description: DNase-seq DEX treatment
Submitted by
Linda Hong
Lab
Tim Reddy, Duke
treatment protocol
Description: RNA-seq_DEX_treatment_protocol
Submitted by
Linda Hong
Lab
Tim Reddy, Duke