ENCBS389TMS / cell line
Summary
- Status
- released
- Term name
- HCT116
- Term ID
- EFO:0002824
- Summary
- Homo sapiens HCT116 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (pgRNA) 14 days after the sample was treated with 2 μg/mL Puromycin for 2 days, 0.2 μg/mL Puromycin for 2 days
- Culture start date
- 2019-03-01
- Culture harvest date
- 2019-04-20
Attribution
ENCODE4 project
- Lab
- Bing Ren, UCSD
- Award PI
- Yin Shen, UCSF
- Submitted by
- Bin Li
- Source
- ATCC
- Project
- ENCODE
- External resources
- Aliases
- bing-ren:BC19B_HCT116_D14_B2_BC79
Treatment details
- Treatment
- 2 μg/mL Puromycin (CHEBI:17939) for 2 days
- Type
- chemical
- Purpose
- selection
- Treatment
- 0.2 μg/mL Puromycin (CHEBI:17939) for 2 days
- Type
- chemical
- Purpose
- selection
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM554RTS | insertion | expression | transduction | ||
| ENCGM353IMQ | CRISPRi | characterization | CRISPR |
Donor information
- Status
- released
- Accession
- ENCDO000ABE
- Aliases
- encode:donor of HCT-116, bradley-bernstein:Donor of HCT-116 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 48 years
- Sex
- male
- Health status
- colorectal carcinoma
- External resources
- References