ENCBS380PDP / whole organisms
Summary
- Status
- released
- Term name
- whole organism
- Term ID
- UBERON:0000468
- Summary
- Drosophila melanogaster strain usp-GFP wandering third instar larva whole organism stably expressing C-terminal eGFP-tagged usp
- Life stage
- wandering third instar larva
- Age
- Unknown
Attribution
modENCODE Project
- Lab
- Kevin White, UChicago
- Award PI
- Kevin White, UChicago
- Submitted by
- Kevin White
- Source
- Kevin White
- Project
- modENCODE
- Aliases
- kevin-white:B1-modencode_4109
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM259IVV | insertion | tagging | stable transfection |
|
Strain information
- Status
- released
- Accession
- ENCDO440VTK
- Aliases
- modencode:usp-GFP, bloomington:38672
- Donor external identifiers
- Species
- Drosophila melanogaster
- Genotype
- w[1118]; PBac{y[+mDint2] w[+mC]=usp-GFP.FLAG}VK00033
- Sex
- unknown
- Strain background
- VK00033
- Strain name
- usp-GFP
- External resources
Documents
immunofluorescence
Caption excerpt: Strain name: usp-GFP
Genotype: PBac{y[+]-attP-3B}VK00033
Gene…
- Caption
- Strain name: usp-GFP Genotype: PBac{y[+]-attP-3B}VK00033 Gene Target: usp Transgene: USP genomic coding region, stop codon replaced with GFP Tag Type: LAP-TAG_EGFP Short Description: P[acman] BAC number CH322-23L04 has been chosen to target the gene ultraspiracle. The LAP-TAG containing the GFP has been recombineered in 3' of the gene in frame. The engineered vector has then been inserted into the specific docking site with the help of Att sites. Recombinant individuals were screened based on mini-white presence. Vector Used: P[acman] Genomic Coordinates: X from 1928252 to 1947898. attP Coordinates: 3L:6435776..6435776 Made_by: Nader Jamel, Alec Victorsen, Rebecca Spokony
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U01HG004264