ENCBS372QTG / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP645 (unc-119(tm4063) III; wgIs645) mixed sex mixed stage (embryo) 2 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-mls-2
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to MLS-2
Life stage
mixed stage (embryonic)
Age
Unknown
Synchronization timepoint
egg bleaching + 2.0 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
Aliases
valerie-reinke:MLS-2_GFP_OP645_EM_Rep1

Genetic modifications

AccessionCategoryPurposeMethodSite
ENCGM510LWVinsertiontaggingbombardment
Coordinates
ce11 chrX:4747377-4782527
Integration site
random

Strain information

Status
released
Accession
ENCDO226NKQ
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063) III; wgIs645
Sex
mixed
Strain name
OP645

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale
general protocol
Description: Protocol used by the Reinke lab for ChIP-seq
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale