ENCBS366PAP / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line genetically modified (insertion) using CRISPR targeting ZNF395
Description
K562 cell line stably expressing a C-terminal LAP-tag containing eGFP, fused to ZNF395
Culture start date
2015-08-17
Culture harvest date
2015-09-19
Passage number
4

Attribution

ENCODE3 project
Lab
Kevin White, UChicago
Award PI
Michael Snyder, Stanford
Submitted by
Alec Victorsen
Project
ENCODE
Aliases
kevin-white:ZNF395_CRISPR_GFP_K562_Rep3

Genetic modifications

Accession
Category
Purpose
Method
Site
ENCGM446NKUinsertiontaggingCRISPR

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
External resources
References

Documents

immunoblot
Caption: immunoblot of GFP tagged ZNF395, expressed in K562 cells
Attachment from submitter
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
general protocol
Description: Protocol for generating GFP tagged transgenic lines in K562 by CRISPR
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago
immunofluorescence
Caption: immunofluorescence of GFP tagged ZNF395 in K562 cells
Attachment from submitter
Submitted by
Alec Victorsen
Lab
Kevin White, UChicago