ENCBS360JZL / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line stably expressing C-terminal eGFP-tagged TAF7
Description
K562 cell line stably expressing a C-terminal LAP-tag containing eGFP, fused to TAF7
Culture start date
2013-11-13
Culture harvest date
2014-04-07
Passage number
5

Attribution

ENCODE3 project
Lab
Kevin White, UChicago
Award PI
Michael Snyder, Stanford
Submitted by
Mark Murphy
Project
ENCODE
Aliases
kevin-white:TAF7_GFP_2
Submitter comment
Biorep2

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM145FXMinsertiontaggingstable transfection

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
External resources
References

Documents

transfection protocol
Description: UChicago IGSB ENCODE K562 Transfection Protocol March 5, 2013
Submitted by
Jay Rehm
Lab
Kevin White, UChicago
FACs analysis
Caption excerpt: eGFP-TAF7 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby…
Attachment from submitter
Caption
eGFP-TAF7 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP+ fraction quantified for their GFP and nuclear signal colocalization.
Submitted by
Mark Murphy
Lab
Kevin White, UChicago
FACs analysis
Caption excerpt: eGFP-TAF7 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby…
Attachment from submitter
Caption
eGFP-TAF7 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP++ fraction quantified for their GFP and nuclear signal colocalization.
Submitted by
Mark Murphy
Lab
Kevin White, UChicago
FACs analysis
Caption excerpt: eGFP-TAF7 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby…
Attachment from submitter
Caption
eGFP-TAF7 tagged cells were stained with a nuclear dye, Vybrant DyeCycle Ruby Stain from Life technologies, and sorted by FACS. Shown are the cells from the GFP+ fraction quantified for their GFP and nuclear signal colocalization.
Submitted by
Mark Murphy
Lab
Kevin White, UChicago