ENCBS346WQD / tissue
Summary
- Status
- released
- Term name
- heart
- Term ID
- UBERON:0000948
- Summary
- Homo sapiens male child (3 years) heart tissue
- Description
- Heart primary tissue from a healthy 3 year old male human from the Lin laboratory.
Attribution
Roadmap Epigenomics Mapping Consortium
- Lab
- John Stamatoyannopoulos, UW
- Award PI
- John Stamatoyannopoulos, UW
- Submitted by
- Marcus Ho
- Source
- Shin Lin
- Project
- Roadmap
- Aliases
- roadmap-epigenomics:Heart.STL001y3, roadmap-epigenomics:Heart_Oct-27-2012_32363
- Submitter comment
- collection_method: After the donor aorta and inferior mesenteric vein (IMV) were cannulated, the aorta was flushed with 5L of 4degC cooled histidine-tryptophan-ketoglutarate solution (HTK) and the IMV was flushed with 3L of HTK which results in the perfusion of all intra-abdominal organs with preservative solution. The abdominal cavity was cooled topically with ice during perfusion. The relevant tissues were then removed and transported to a local laboratory within 1 hour. There, the tissues were minced with a blade and 1gm of tissue was placed into a cryovial with 1mL of freezing solution (freezing solution 50mL: 25mL DMEM, 10mL Normosol-R, 10mL 25% BSA, 5mL DMSO, 1 tablet protease inhibitor) for 15min on ice. The cyrovial was then placed in a 1inch-thick styrofoam container, and then placed into -80C freezer, resulting in a slow freezing to -80C over an 1hour period.
Donor information
- Status
- released
- Accession
- ENCDO478OMA
- Aliases
- roadmap-epigenomics:STL001_donor
- Species
- Homo sapiens
- Life stage
- child
- Age
- 3 years
- Sex
- male
- Health status
- healthy