ENCBS334WBP / cell line
Summary
- Status
- released
- Term name
- HepG2
- Term ID
- EFO:0001187
- Summary
- Homo sapiens HepG2 cell line expressing RNAi targeting H. sapiens PRPF4
- Description
- RNA-seq on HepG2 cells treated with an shRNA knockdown against PRPF4. (PRPF4-BGHLV37-42B)
- Product ID
- HB-8065
- Lot ID
- 59635738
- Culture start date
- 2016-08-25
- Culture harvest date
- 2016-09-07
Attribution
ENCODE3 project
- Lab
- Brenton Graveley, UConn
- Award PI
- Brenton Graveley, UConn
- Submitted by
- Xintao Wei
- Source
- ATCC
- Project
- ENCODE
- External resources
- Aliases
- brenton-graveley:PRPF4-BGHLV37-42B
- Tags
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM459UVO | interference | repression | RNAi | transduction |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAC
- Aliases
- encode:donor of HepG2, bradley-bernstein:Donor of HepG2
- Species
- Homo sapiens
- Life stage
- child
- Age
- 15 years
- Sex
- male
- Health status
- hepatocellular carcinoma
- Ethnicity
- European
- External resources
- References
Documents
general protocol
Description: Biosample protocol for PRPF4-BGHLV37-42B
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
immunoblot
Caption excerpt: Western blot following shRNA against PRPF4 in HepG2 whole cell lysate using…
- Caption
- Western blot following shRNA against PRPF4 in HepG2 whole cell lysate using PRPF4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PRPF4.PRPF4 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005