ENCBS303WCA / in vitro differentiated cells

Summary

Status
released
Term name
hematopoietic multipotent progenitor cell
Term ID
CL:0000837
Summary
Homo sapiens male adult (25 years) hematopoietic multipotent progenitor in vitro differentiated cells treated with interleukin-3 for 18 days, kit ligand for 18 days, hydrocortisone succinate for 18 days, erythropoietin for 18 days
Description
Day 18 (T18)

Attribution

ENCODE3 project
Lab
John Stamatoyannopoulos, UW
Award PI
John Stamatoyannopoulos, UW
Submitted by
Michael Buckley
Project
ENCODE
External resources
Aliases
john-stamatoyannopoulos:DS25969A

Treatment details

Treatment
interleukin-3 (UniProtKB:P08700) for 18 day
Type
protein
Treatment
kit ligand (UniProtKB:P21583) for 18 day
Type
protein
Treatment
hydrocortisone succinate (CHEBI:31677) for 18 day
Type
chemical
Treatment
erythropoietin (CHEBI:81579) for 18 day
Type
chemical

Donor information

Status
released
Accession
ENCDO937OUY
Aliases
encode:donor of RO 02366, roadmap-epigenomics:RO 02366, john-stamatoyannopoulos:RO_02366
Donor external identifiers
Species
Homo sapiens
Life stage
adult
Age
25 years
Sex
male

Documents

treatment protocol
Description: Erythroid differentiation of human CD34+ cells
Submitted by
Jason Hilton
Lab
John Stamatoyannopoulos, UW
cell isolation protocol
Description: Isolation of CD34-positive cells from human leukapheresis product
Submitted by
Michael Buckley
Lab
John Stamatoyannopoulos, UW