ENCBS266WUA / cell line
Summary
- Status
- released
- Term name
- K562
- Term ID
- EFO:0002067
- Summary
- Homo sapiens K562 cell line genetically modified (insertion) using CRISPR targeting H. sapiens USF2
- Description
- K562 cell line stably expressing a C-terminal LAP-tag containing eGFP, fused to USF2
- Culture start date
- 2016-01-07
- Culture harvest date
- 2016-02-09
- Passage number
- 4
Attribution
ENCODE3 project
- Lab
- Kevin White, UChicago
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Alec Victorsen
- Source
- Kevin White
- Project
- ENCODE
- External resources
- Aliases
- kevin-white:USF2_CRISPR_GFP_K562_Rep1
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM042TVT | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAD
- Aliases
- encode:donor of K562, bradley-bernstein:Donor of K562 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- female
- Health status
- chronic myelogenous leukemia (CML)
- External resources
- References
Documents
immunoblot
Caption: immunoblot of GFP tagged USF2, expressed in K562 cells
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996
general protocol
Description: Protocol for generating GFP tagged transgenic lines in K562 by CRISPR
- Submitted by
- Alec Victorsen
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996