ENCBS253FYV / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP139 (wgIs139(ceh-27::TY1 EGFP 3xFLAG(P000007_F10); unc-119(+));unc-119(ed3)) mixed sex young adult (73 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans ceh-27
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to CEH-27.
Life stage
young adult
Age
73 hours
Synchronization timepoint
L1 larva starved after bleaching

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:CEH-27_GFP_OP139_yAd_INPUT2

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM959ATTinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO518ZHE
Aliases
robert-waterston:OP139
Species
Caenorhabditis elegans
Genotype
wgIs139(ceh-27::TY1 EGFP 3xFLAG(P000007_F10); unc-119(+));unc-119(ed3)
Sex
mixed
Strain name
OP139
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale