ENCBS223ROE / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP756 (unc-119(tm4063); wgIs756 [ceh-13::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex early embryo (2 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-ceh-13
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to CEH-13.
Life stage
early embryonic
Age
2 hours
Synchronization timepoint
egg bleaching

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
Aliases
valerie-reinke:CEH-13_GFP_OP756_EE_INPUT1

Genetic modifications

AccessionCategoryPurposeMethodSite
ENCGM130FKKinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO569IUL
Aliases
cgc:OP756
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063); wgIs756 [ceh-13::TY1::EGFP::3xFLAG + unc-119(+)]
Sex
mixed
Strain name
OP756

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale