ENCBS221YFO / cell line

Summary

Status
released
Term name
K562
Summary
Homo sapiens K562 cell line expressing RNAi
Description
RNA-seq on K562 cells treated with an shRNA knockdown against Nontarget. (non-target_BGKLV28-2)
Product ID
CCL-243
Lot ID
59300853
Culture start date
2015-06-11
Culture harvest date
2015-06-22

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award PI
Brenton Graveley, UConn
Submitted by
Xintao Wei
Source
ATCC
Project
ENCODE
Aliases
brenton-graveley:non-target_BGKLV28-2
Tags
Visit collection page for ENCORE

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM266AWPinterferencenon-specific target controlRNAitransduction

Donor information

Status
released
Accession
ENCDO000AAD
Aliases
encode:donor of K562, bradley-bernstein:Donor of K562 cells
Species
Homo sapiens
Life stage
adult
Age
53 years
Sex
female
Health status
chronic myelogenous leukemia (CML)
External resources
References

Documents

growth protocol
Description: Growth protocol for K562 cells used in the RNA evaluation dataset for ENCODE
Submitted by
Brenton Graveley
Lab
Brenton Graveley, UConn
general protocol
Description excerpt: Graveley Lab shRNA knockdown followed by RNA-seq Biosample Preparation and…
Description
Graveley Lab shRNA knockdown followed by RNA-seq Biosample Preparation and Characterization Document
Submitted by
Julie Wong
Lab
Brenton Graveley, UConn