ENCBS201PBE / whole organisms
Summary
- Status
- released
- Term name
- whole organism
- Term ID
- UBERON:0000468
- Summary
- Caenorhabditis elegans strain LW1254 (jjIs1254[Im486.2: pJKL723.2(sma-9C2::gfp+unc-119(+) ]; unc-119(ed4)) hermaphrodite L2 larva 22 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans sma-9
- Description
- Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to SMA-9
- Life stage
- L2 larva
- Age
- Unknown
- Synchronization timepoint
- L1 larva starved after bleaching + 22.0 hours
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award PI
- Robert Waterston, UW
- Submitted by
- Alec Victorsen
- Source
- Valerie Reinke
- Project
- modERN
- External resources
- Aliases
- valerie-reinke:SMA-9_GFP_LW1254_L2_INPUT
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
---|---|---|---|---|---|
ENCGM563VNW | insertion | tagging | bombardment |
|
Strain information
- Status
- released
- Accession
- ENCDO730ORW
- Donor external identifiers
- Species
- Caenorhabditis elegans
- Genotype
- jjIs1254[Im486.2: pJKL723.2(sma-9C2::gfp+unc-119(+) ]; unc-119(ed4)
- Sex
- hermaphrodite
- Strain name
- LW1254
- External resources
Documents
immunofluorescence
- Caption
- DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
- Submitted by
- Alec Victorsen
- Lab
- Valerie Reinke, Yale
- Grant
- U41HG007355