ENCBS201PBE / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain LW1254 (jjIs1254[Im486.2: pJKL723.2(sma-9C2::gfp+unc-119(+) ]; unc-119(ed4)) hermaphrodite L2 larva 22 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans sma-9
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to SMA-9
Life stage
L2 larva
Age
Unknown
Synchronization timepoint
L1 larva starved after bleaching + 22.0 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:SMA-9_GFP_LW1254_L2_INPUT

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM563VNWinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO730ORW
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
jjIs1254[Im486.2: pJKL723.2(sma-9C2::gfp+unc-119(+) ]; unc-119(ed4)
Sex
hermaphrodite
Strain name
LW1254
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale