ENCBS192ZMY / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP639 (wgIs639 [ham-2::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex mixed stage (embryo) (3 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans ham-2
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to HAM-2.
Life stage
mixed stage (embryonic)
Age
3 hours
Synchronization timepoint
egg bleaching

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:HAM-2_GFP_OP639_EM_INPUT6

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM204KRBinsertiontaggingbombardment
Integration site
random

Strain information

Status
released
Accession
ENCDO199MIC
Aliases
robert-waterston:OP639
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
wgIs639 [ham-2::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)
Sex
mixed
Strain name
OP639
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale