ENCBS179YEH / cell line
Summary
- Status
- released
- Term name
- A549
- Term ID
- EFO:0001086
- Summary
- Homo sapiens A549 cell line genetically modified (insertion) using CRISPR targeting H. sapiens ATOH8
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Nathaniel Watson
- Source
- Michael Snyder
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:pB-2584, michael-snyder:pB-eGFP-ATOH8 A549 input
Pooled from biosamples
2 biosamples
Accession | Type | Term | Summary |
|---|---|---|---|
| ENCBS340ICI | cell line | A549 | Homo sapiens A549 cell line genetically modified (insertion) using CRISPR targeting H. sapiens ATOH8 |
| ENCBS945LTN | cell line | A549 | Homo sapiens A549 cell line genetically modified (insertion) using CRISPR targeting H. sapiens ATOH8 |
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM994XBD | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAZ
- Aliases
- encode:donor of A549, bradley-bernstein:Donor of A549 cells, john-stamatoyannopoulos:ATCC_A549
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 58 years
- Sex
- male
- Ethnicity
- European
- External resources
- References
Documents
growth protocol
Description: Cell growth protocol for A549 cells (version 1).
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
transfection protocol
Description excerpt: A549 transfection protocol - Version 1 (Replicates split right after…
- Description
- A549 transfection protocol - Version 1 (Replicates split right after transfection, 500 µg/ml G418)
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996