ENCBS173VLG / primary cell
Summary
- Status
- released
- Term name
- stimulated activated naive CD8-positive, alpha-beta T cell
- Term ID
- NTR:0000667
- Summary
- Homo sapiens male adult (42 years) stimulated activated naive CD8-positive, alpha-beta T primary cell treated with anti-CD3 and anti-CD28 coated beads for 24 hours, 50 U/mL Interleukin-2 for 24 hours, 100 ng/mL Interleukin-15 for 24 hours
- Parent of biosamples
- ENCBS092GXN, ENCBS661KXZ, ENCBS442TID, ENCBS337FXX, ENCBS819MUY
Attribution
ENCODE4 project
- Lab
- John Stamatoyannopoulos, UW
- Award PI
- John Stamatoyannopoulos, UW
- Submitted by
- Michael Buckley
- Source
- AllCells
- Project
- ENCODE
- Aliases
- john-stamatoyannopoulos:TC14469
Treatment details
- Treatment
- anti-CD3 and anti-CD28 coated beads for 24 hours
- Type
- antibody
- Purpose
- activation
- Treatment
- 50 U/mL Interleukin-2 (UniProtKB:P60568) for 24 hours
- Type
- protein
- Purpose
- activation
- Treatment
- 100 ng/mL Interleukin-15 (UniProtKB:P40933) for 24 hours
- Type
- protein
- Purpose
- stimulation
Donor information
- Status
- released
- Accession
- ENCDO833KUM
- Donor external identifiers
- AllCells:6054
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 42 years
- Sex
- male
- External resources
Documents
cell isolation protocol
Description excerpt: Isolation of Immune cells by Stemcell Technologies Magnetic Beads kits and…
- Description
- Isolation of Immune cells by Stemcell Technologies Magnetic Beads kits and stimulations (Stamatoyannopoulos lab). This protocol is an updated version of : https://www.encodeproject.org/documents/b94d1a22-1ef2-4820-9b71-cdcfba41127c/
- Submitted by
- Jennifer Jou
- Lab
- John Stamatoyannopoulos, UW
- Grant
- UM1HG009444