ENCBS134ZWP / cell line

Summary

Status
released
Term name
WW6
Summary
Mus musculus strain ~20% C57/B6J (MGI.D:C57BL), ~75% 129/Sv (MGI.D:129) and ~5% SJL strains (MGI.D:SJL) WW6 cell line genetically modified (knockout) using homologous recombination targeting M. musculus Hist1h1c, genetically modified (knockout) using homologous recombination targeting M. musculus Hist1h1d, genetically modified (knockout) using homologous recombination targeting M. musculus Hist1h1e
Description
Histone H1c, H1d, H1e triple null mouse embryonic stem cell line derived from ES-WW6 cells.
Life stage
embryonic
Age
Unknown

Attribution

ENCODE Mouse Project
Lab
John Stamatoyannopoulos, UW
Award PI
John Stamatoyannopoulos, UW
Submitted by
John Stamatoyannopoulos
Project
ENCODE
Aliases
john-stamatoyannopoulos:DS18365

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM993OPNknockoutrepressionhomologous recombination
ENCGM387XZXknockoutrepressionhomologous recombination
ENCGM245FBVknockoutrepressionhomologous recombination

Strain information

Status
released
Accession
ENCDO078AAA
Aliases
encode:WW6-strain-mix
Species
Mus musculus
Sex
male
Health status
unknown
Strain background
mixed
Strain name
~20% C57/B6J (MGI.D:C57BL), ~75% 129/Sv (MGI.D:129) and ~5% SJL strains (MGI.D:SJL)

Documents

growth protocol
Description excerpt: Propagation of wild-type and triple-H1-null mouse embryonic stem cells…
Description
Propagation of wild-type and triple-H1-null mouse embryonic stem cells protocol (Skoultchi Lab)
Submitted by
John Stamatoyannopoulos
Lab
John Stamatoyannopoulos, UW