ENCBS134HRW / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP640 (unc-119(tm4063) III; wgIs640) mixed sex early embryo using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans tra-4
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to TRA-4
Life stage
early embryonic
Age
Unknown
Synchronization timepoint
egg bleaching

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:TRA-4_GFP_OP640_EE_INPUT

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM376RCIinsertiontaggingbombardment
Coordinates
ce11 chrX:2840685-2875481
Integration site
random

Strain information

Status
released
Accession
ENCDO583OPC
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063) III; wgIs640.
Sex
mixed
Strain name
OP640
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale