ENCBS111IHC / cell line

Summary

Status
released
Term name
HepG2
Summary
Homo sapiens HepG2 cell line genetically modified (deletion) using CRISPR targeting H. sapiens SRSF7
Description
RNA-seq on HepG2 cells treated with a CRISPR gRNA against SRSF7. (SRSF7_BGHcLV03-5)
Product ID
HB-8065
Lot ID
59635738

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award PI
Brenton Graveley, UConn
Submitted by
Xintao Wei
Source
ATCC
Project
ENCODE
Aliases
brenton-graveley:SRSF7_BGHcLV03-5
Tags
Visit collection page for ENCORE

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM146LLTdeletionrepressionCRISPR

Donor information

Status
released
Accession
ENCDO000AAC
Aliases
encode:donor of HepG2, bradley-bernstein:Donor of HepG2
Species
Homo sapiens
Life stage
child
Age
15 years
Sex
male
Health status
hepatocellular carcinoma
Ethnicity
European
External resources
References

Documents

growth protocol
Description: Growth protocol for HepG2
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
general protocol
Description excerpt: Graveley Lab CRISPR Genome Editing followed by RNA-seq Biosample Preparation…
Description
Graveley Lab CRISPR Genome Editing followed by RNA-seq Biosample Preparation and Characterization Document
Submitted by
Zachary Myers
Lab
Brenton Graveley, UConn