ENCBS076AAA / cell line
Summary
- Status
- released
- Term name
- K562
- Term ID
- EFO:0002067
- Summary
- Homo sapiens K562 cell line stably expressing C-terminal eGFP-tagged ILK
- Description
- K562 cell line stably expressing a C-terminal LAP-tag containing eGFP, fused to ILK
- Culture harvest date
- 2012-12-19
- Passage number
- 4
Attribution
ENCODE3 project
- Lab
- Kevin White, UChicago
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Jay Rehm
- Source
- Kevin White
- Project
- ENCODE
- External resources
- Aliases
- kevin-white:ILK_R1
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM593WHV | insertion | tagging | stable transfection |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAD
- Aliases
- encode:donor of K562, bradley-bernstein:Donor of K562 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- female
- Health status
- chronic myelogenous leukemia (CML)
- External resources
- References
Documents
transfection protocol
Description: UChicago IGSB ENCODE K562 Transfection Protocol March 5, 2013
- Submitted by
- Jay Rehm
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996
immunoblot
Caption excerpt: Western blot analysis of nuclear lysates prepared from a K562 stable cell line…
- Caption
- Western blot analysis of nuclear lysates prepared from a K562 stable cell line expressing eGFP-ILK using the Roche anti-GFP 11814450001 antibody. The expected MW is 85kDa.
- Submitted by
- Jay Rehm
- Lab
- Kevin White, UChicago
- Grant
- U54HG006996