ENCBS075TJY / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP658 (unc-119(tm4063) III; wgIs658) mixed sex young adult 63 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans F10E7.11
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to F10E7.11
Life stage
young adult
Age
Unknown
Synchronization timepoint
L1 larva starved after bleaching + 63.0 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:F10E7.11_GFP_OP658_yAd_INPUT

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM507SDWinsertiontaggingbombardment
Coordinates
ce11 chrII:7103263-7133096
Integration site
random

Strain information

Status
released
Accession
ENCDO001ODU
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063) III; wgIs658.
Sex
mixed
Strain name
OP658
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale