ENCBS074BKM / cell line

Summary

Status
released
Term name
A549
Summary
Homo sapiens A549 cell line genetically modified (insertion) using CRISPR targeting H. sapiens HOXA7
Separated from biosample
ENCBS830VTV
Parent of biosamples
ENCBS801NYS

Attribution

ENCODE4 project
Lab
Michael Snyder, Stanford
Award PI
Michael Snyder, Stanford
Submitted by
Nathaniel Watson
Project
ENCODE
External resources
Aliases
michael-snyder:pB-11154, michael-snyder:pB-eGFP-HOXA7 A549 (batch 2)

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM243DSDinsertiontaggingCRISPR

Donor information

Status
released
Accession
ENCDO000AAZ
Aliases
encode:donor of A549, bradley-bernstein:Donor of A549 cells, john-stamatoyannopoulos:ATCC_A549
Species
Homo sapiens
Life stage
adult
Age
58 years
Sex
male
Ethnicity
European
External resources
References

Documents

growth protocol
Description: Cell growth protocol for A549 cells (version 1).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
transfection protocol
Description excerpt: A549 transfection protocol - Version 1 (Replicates split right after…
Description
A549 transfection protocol - Version 1 (Replicates split right after transfection, 500 µg/ml G418)
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford