ENCBS066XFJ / whole organisms
Summary
- Status
- revoked
- Term name
- whole organism
- Term ID
- UBERON:0000468
- Summary
- Caenorhabditis elegans strain OP494 (unc-119(tm4063) III; wgIs494) hermaphrodite late embryo 5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans ztf-16
- Description
- Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to ZTF-16
- Life stage
- late embryonic
- Age
- Unknown
- Synchronization timepoint
- egg bleaching + 5 hours
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award PI
- Robert Waterston, UW
- Submitted by
- Alec Victorsen
- Source
- Valerie Reinke
- Project
- modERN
- Aliases
- valerie-reinke:ZTF-16_GFP_OP494_LE_Rep2
- Submitter comment
- The submitting lab requested to revoke the data associated with strain OP494 because genotyping revealed that strain contains both tagged ztf-16 and lag-1.
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM642POY | insertion | tagging | bombardment |
|
Strain information
- Status
- revoked
- Accession
- ENCDO421TEB
- Donor external identifiers
- Species
- Caenorhabditis elegans
- Genotype
- unc-119(tm4063) III; wgIs494.
- Sex
- hermaphrodite
- Strain name
- OP494
Documents
immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
- Caption
- DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
- Submitted by
- Alec Victorsen
- Lab
- Valerie Reinke, Yale
- Grant
- U41HG007355