ENCBS066ENC / cell line
Summary
- Status
- released
- Term name
- MCF 10A
- Term ID
- EFO:0001200
- Summary
- Homo sapiens MCF 10A cell line
- Description
- mammary gland, non-tumorigenic epithelial, inducible cell line, derived from the MCF-10A parental cells and contain ER-Src, a derivative of the Src kinase oncoprotein (v-Src) that is fused to the ligand-binding domain of the estrogen receptor (ER); To generate v-Src:ER, DNA sequences encoding the Schmidt Ruppin A form of v-Src (a gift from Josh Kaplan) were fused to hbER and cloned into the pWZLblast3, pBabepuro3, or pLNCX retroviral expression vectors. In the case of pWZLblast3 (a gift from Jay Morgenstern), expression of a bicistronic mRNA from the murine leukemia virus long terminal repeat containing the encephalomyocarditis virus internal ribosome entry site permits the expression of both v-Src:ER and the blasticidin drug resistance gene - from PMID:9891045.
Attribution
ENCODE2 project
- Lab
- Kevin Struhl, HMS
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Kevin Struhl
- Source
- Kevin Struhl
- Project
- ENCODE
- External resources
Donor information
- Status
- released
- Accession
- ENCDO000AAP
- Aliases
- encode:donor of MCF10A-Er-Src, encode:donor of MCF 10A
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 36 years
- Sex
- female
- Health status
- fibrocystic disease
- Ethnicity
- European
- External resources
- References