ENCBS066ENC / cell line

Summary

Status
released
Term name
MCF 10A
Summary
Homo sapiens MCF 10A cell line
Description
mammary gland, non-tumorigenic epithelial, inducible cell line, derived from the MCF-10A parental cells and contain ER-Src, a derivative of the Src kinase oncoprotein (v-Src) that is fused to the ligand-binding domain of the estrogen receptor (ER); To generate v-Src:ER, DNA sequences encoding the Schmidt Ruppin A form of v-Src (a gift from Josh Kaplan) were fused to hbER and cloned into the pWZLblast3, pBabepuro3, or pLNCX retroviral expression vectors. In the case of pWZLblast3 (a gift from Jay Morgenstern), expression of a bicistronic mRNA from the murine leukemia virus long terminal repeat containing the encephalomyocarditis virus internal ribosome entry site permits the expression of both v-Src:ER and the blasticidin drug resistance gene - from PMID:9891045.

Attribution

ENCODE2 project
Lab
Kevin Struhl, HMS
Award PI
Michael Snyder, Stanford
Submitted by
Kevin Struhl
Project
ENCODE

Donor information

Status
released
Accession
ENCDO000AAP
Aliases
encode:donor of MCF10A-Er-Src, encode:donor of MCF 10A
Species
Homo sapiens
Life stage
adult
Age
36 years
Sex
female
Health status
fibrocystic disease
Ethnicity
Caucasian
References

Documents

growth protocol
Description: MCF10A-Er-Src
Submitted by
Kevin Struhl
Lab
Kevin Struhl, HMS