ENCBS061VIU / cell line

Summary

Status
released
Term name
HCT116
Summary
Homo sapiens HCT116 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (pgRNA) 0 days after the sample was treated with 2 μg/mL Puromycin for 2 days, 0.2 μg/mL Puromycin for 2 days
Culture start date
2019-03-01
Culture harvest date
2019-04-20

Attribution

ENCODE4 project
Lab
Bing Ren, UCSD
Award PI
Yin Shen, UCSF
Submitted by
Bin Li
Source
ATCC
Project
ENCODE
External resources
Aliases
bing-ren:BC19B_HCT116_D0_B2_BC75

Treatment details

Treatment
2 μg/mL Puromycin (CHEBI:17939) for 2 days
Type
chemical
Purpose
selection
Treatment
0.2 μg/mL Puromycin (CHEBI:17939) for 2 days
Type
chemical
Purpose
selection

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM554RTSinsertionexpressiontransduction
ENCGM353IMQCRISPRicharacterizationCRISPR

Donor information

Status
released
Accession
ENCDO000ABE
Aliases
encode:donor of HCT-116, bradley-bernstein:Donor of HCT-116 cells
Species
Homo sapiens
Life stage
adult
Age
48 years
Sex
male
Health status
colorectal carcinoma
External resources
References