ENCBS039CPR / cell line
Summary
- Status
- released
- Term name
- HepG2
- Term ID
- EFO:0001187
- Summary
- Homo sapiens HepG2 cell line genetically modified (insertion) using CRISPR targeting H. sapiens ATF4
- Description
- HepG2 cell line stably expressing a C-terminal LAP-tag containing eGFP, fused to ATF4.
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Nathaniel Watson
- Source
- Michael Snyder
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:HepG2_eGFP-ATF4_CRISPR_ChIP_B1
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM865NGH | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAC
- Aliases
- encode:donor of HepG2, bradley-bernstein:Donor of HepG2
- Species
- Homo sapiens
- Life stage
- child
- Age
- 15 years
- Sex
- male
- Health status
- hepatocellular carcinoma
- Ethnicity
- European
- External resources
- References
Documents
immunoblot
Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…
- Caption
- Immunoprecipitation was performed on nuclear extracts from biosample ENCBS050IJL (HepG2 eGFP-ATF4 replicate 2) cells using anti-eGFP antibody. The image shows western blot analysis of input (lane 1), immunoprecipitate (lane 2), and mock immunoprecipitate using IgG (lane 3). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD). The target molecular weight is 72.0 kD as indicated with an arrow.
- Submitted by
- Ingrid Youngworth
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442