ENCBS038REP / cell line

Summary

Status
released
Term name
SK-N-SH
Summary
Homo sapiens SK-N-SH cell line genetically modified (insertion) using CRISPR targeting H. sapiens GLIS3 treated with 6 μM all-trans-retinoic acid for 48 hours
Culture harvest date
2018-09-26
Separated from biosample
ENCBS651SCV

Attribution

ENCODE4 project
Lab
Michael Snyder, Stanford
Award PI
Michael Snyder, Stanford
Submitted by
Nathaniel Watson
Project
ENCODE
Aliases
michael-snyder:pB-11121, michael-snyder:pB-eGFP-GLIS3 SK-N-SH rep8

Treatment details

Treatment
6 μM all-trans-retinoic acid (CHEBI:15367) for 48 hours
Type
chemical
Purpose
activation

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM708KYJinsertiontaggingCRISPR

Donor information

Status
released
Accession
ENCDO000ABD
Aliases
encode:donor of SK-N-SH, bradley-bernstein:Donor of SK-N-SH cells, encode:donor of SH-SY5Y
Species
Homo sapiens
Life stage
child
Age
4 years
Sex
female
Health status
Metastatic neuroblastoma from bone marrow
External resources
References

Documents

immunoblot
Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from biosample ENCBS071NZE (SK-N-SH eGFP-GLIS3 replicate 1) cells using anti-eGFP antibody. The image shows western blot analysis of input (lane 1), immunoprecipitate (lane 2), and mock immunoprecipitate using IgG (lane 3). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD). The target molecular weight is 112.0 kD as indicated with an arrow.
Submitted by
Jessica Au
Lab
Michael Snyder, Stanford
Submitter comment
DCC submitted redundant biosample characterization based off of this biosample-characterizations/536f2fd5-e6e5-4721-9ee0-f5ebdc5d3cba/
growth protocol
Description: Cell growth protocol for SK-N-SH cells (version 1).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
transfection protocol
Description excerpt: SK-N-SH transfection protocol - Version 1 (Replicates split at T-75 stage, 200…
Description
SK-N-SH transfection protocol - Version 1 (Replicates split at T-75 stage, 200 µg/ml G418)
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
treatment protocol
Description: RA treatment protocol for SK-N-SH cells (version 1).
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford