ENCBS032PQC / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP573 (unc-119(tm4063) III; wgIs573) mixed sex early embryo 1.5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans hnd-1
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to HND-1
Life stage
early embryonic
Age
Unknown
Synchronization timepoint
egg bleaching + 1.5 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:HND-1_GFP_OP573_EE_INPUT

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM856QXUinsertiontaggingbombardment
Coordinates
ce11 chrX:11664914-11698412
Integration site
random

Strain information

Status
released
Accession
ENCDO147KSU
Aliases
robert-waterston:OP573
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063) III; wgIs573
Sex
mixed
Strain name
OP573
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale
general protocol
Description: Protocol used by the Reinke lab for ChIP-seq
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale