ENCBS023OFD / whole organisms

Summary

Status
released
Term name
whole organism
Summary
Caenorhabditis elegans strain OP696 (unc-119(tm4063) III; wgIs696) mixed sex mixed stage (embryo) 2 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans M03D4.4
Description
Transgenic C. elegans line, stably expressing a C-terminal GFP-FLAG tag fused to M03D4.4
Life stage
mixed stage (embryonic)
Age
Unknown
Synchronization timepoint
egg bleaching + 2.0 hours

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award PI
Robert Waterston, UW
Submitted by
Alec Victorsen
Project
modERN
External resources
Aliases
valerie-reinke:M03D4.4_GFP_OP696_EM_Rep1

Genetic modifications

Accession
Category
Purpose
Method
Nucleic acid delivery method
Site
ENCGM697AGOinsertiontaggingbombardment
Coordinates
ce11 chrIV:6106931-6141310
Integration site
random

Strain information

Status
released
Accession
ENCDO645BPC
Donor external identifiers
Species
Caenorhabditis elegans
Genotype
unc-119(tm4063) III; wgIs696.
Sex
mixed
Strain name
OP696
External resources

Documents

immunofluorescence
Caption excerpt: DIC and GFP image of transgenic worm taken right before collection. Worms…
Attachment from submitter
Caption
DIC and GFP image of transgenic worm taken right before collection. Worms imaged using a Zeiss Axioplan 2 imaging epifluorescence microscope. Scale bars are 10 micron.
Submitted by
Alec Victorsen
Lab
Valerie Reinke, Yale