ENCBS012RXK / cell line
Summary
- Status
- released
- Term name
- GM23338
- Term ID
- EFO:0007950
- Summary
- Homo sapiens GM23338 cell line genetically modified (insertion) using CRISPR targeting H. sapiens CREB1
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Nathaniel Watson
- Source
- Michael Snyder
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:pB-2620, michael-snyder:pB-eGFP-CREB1 PGP1 input
Pooled from biosamples
2 biosamples
Accession | Type | Term | Summary |
|---|---|---|---|
| ENCBS883HUT | cell line | GM23338 | Homo sapiens GM23338 cell line genetically modified (insertion) using CRISPR targeting H. sapiens CREB1 |
| ENCBS299EEL | cell line | GM23338 | Homo sapiens GM23338 cell line genetically modified (insertion) using CRISPR targeting H. sapiens CREB1 |
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM827WTQ | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO336AAA
- Aliases
- bradley-bernstein:Donor hu43860C, encode:donor of hu43860C
- Donor external identifiers
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 53 years
- Sex
- male
- Ethnicity
- European
- External resources
Documents
growth protocol
Description: Cell growth protocol for PGP1 cells (version 1).
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
transfection protocol
Description excerpt: PGP1 transfection protocol - Version 1 (Replicates split right after…
- Description
- PGP1 transfection protocol - Version 1 (Replicates split right after transfection, 30 µg/ml G418, mTeSR medium)
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996