ENCAB997CJG

Alternate accession: ENCAB038PAT

Antibody against Homo sapiens ETV6

Homo sapiens
K562, GM12878
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
HepG2, HeLa-S3
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-166835
Lot ID
B1711
Characterized targets
ETV6 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Isotype
IgG1
Antigen description
raised against amino acids 119-332 of TEL of human origin
Aliases
michael-snyder:AS-285
External resources

Characterizations

ETV6 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-166835. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 57.0
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ETV6 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody sc-166835. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 57.0
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
ETV6 (Homo sapiens)
GM12878K562HeLa-S3HepG2
Method: immunoblot
Attachment from submitter
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HeLaS3, HepG2 using the antibody sc-166835. Molecular Weight: 57.0
Reviewer comment
Multiple bands in HeLa-S3 and HepG2 lanes that would require a knockdown rescue to be compliant.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
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ETV6 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 whole cell lysates using sc-166835, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was silver-stained, gel fragments corresponding to the bands indicated were excised and destained using the SilverSNAP Stain for Mass Spectrometry (Pierce). Then proteins were treated with trypsin using the in-gel digestion method. Digested proteins were analyzed on a LTQ-Orbitrap (Thermo Scientific) b the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (protein false discovery rate <1%, 2 peptides per protein minimum).
Reviewer comment
Excised band shows factor of interest but several other immunoreactive bands were not analyzed.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
ETV6 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
Immunoprecipitation of ETV6 from K562 cells using sc-166835. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with sc-166835. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. Expected band size ~57kD.
Submitter comment
We cut the band based on the size, it's hard to be exact.
Reviewer comment
How come not all immunoreactive bands were excised for mass-spec analysis? ETV6 is detected in the excised band though.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
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