ENCAB996IKB

Homo sapiens

K562

characterized to standards

Homo sapiens

any cell type or tissue

partially characterized

Method: knockdown or knockout

compliant

Caption: Western blot following shRNA against DDX47 in K562 and HepG2 whole cell lysate using DDX47 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX47. Lanes 5-8 follow the same pattern, but in HepG2. DDX47 protein appears as the green band, GAPDH serves as a control and appears in red.
Submitted by: Xintao Wei
Lab: Brenton Graveley, UConn
Grant: U54HG007005
Download: DDX47_Secondary_Western.png
Documents: encode:Antibody_Characterization_ENCODE3_August2015.pdf

Method: immunoprecipitation

not submitted for review by lab

Caption: IP-WB characterization of DDX47 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-DDX47 antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by: Balaji Sundararaman
Lab: Gene Yeo, UCSD
Grant: U54HG007005
Download: GeneTex_GTX120497_40387_DDX47.png

K562

Method: immunoprecipitation

compliant

Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX47 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal Anti-DDX47 antibody (lanes under 'DDX47').
Submitted by: Balaji Sundararaman
Lab: Gene Yeo, UCSD
Grant: U54HG007005
Download: K562_genetex_GTX120497_40387_DDX47.jpg
Documents: encode:Antibody_Characterization_ENCODE3_August2015.pdf
encode:Antibody_Characterization_ENCODE3_February2014.pdf