1. Antibodies
  2. Homo sapiens
  3. STAU2

ENCAB995HZA

Antibody against Homo sapiens STAU2

Homo sapiens
HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
MBLI
Product ID
RN013P
Lot ID
001
Characterized targets
STAU2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
Peptide, 379-396 aa
Antigen sequence
TNLQDQLEKTGENKGWSG
External resources

Characterizations

STAU2 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using STAU2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-STAU2 antibody (lanes under 'STAU2').
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
STAU2 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Representative image of immunoprecipitation performed on whole cell extracts from the HepG2 cell line using the STAU2-specific antibody RN013P. Lane 1: Input from IP using control IgG. Lane 2: Immunoprecipitated material using control IgG. Lane 3: Input from IP using STAU2 antibody. Lane 4: Immunoprecipitated material using STAU2 antibody. Outlined regions were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 62.64 kDa.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
STAU2 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
IP-WB analysis of K562 whole cell lysate using STAU2 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal Anti-STAU2(Human)pAb. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
Submitted by
Balaji Sundararaman
Lab
Gene Yeo, UCSD
STAU2 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Protein was immunoprecipitated from HepG2 whole cell lysates using the antibody RN013P, loaded on a 4-12% NuPAGE Bis-Tris gel, and separated via electrophoresis. Using a reference western blot done in parallel, gel pieces corresponding to the sections indicated were excised and submitted for analysis by the UCSD Biomolecular and Proteomics Mass Spectrometry Facility.
Submitted by
Steven Blue
Lab
Gene Yeo, UCSD
Download
 STAU2 HepG2.pdf
STAU2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against STAU2 in HepG2 whole cell lysate using STAU2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against STAU2.STAU2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
STAU2 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot following CRISPR against STAU2 in K562 whole cell lysate using STAU2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against STAU2. STAU2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn