ENCAB976BHH

Alternate accession: ENCAB000AYI

Antibody against Homo sapiens SMAD2

Homo sapiens
K562, MCF-7, HepG2
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
HeLa, IMR-90, GM12878, HeLa-S3, A549, heart
not characterized to standards
Status
released
Source (vendor)
Rockland
Product ID
600-401-A59
Lot ID
19462
Characterized targets
SMAD2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Aliases
michael-snyder:AS-832
External resources

Characterizations

SMAD2 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody 600-401-A59. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected Molecular Weight: 62 KD
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
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SMAD2 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody 600-401-A59. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
SMAD2 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody 600-401-A59. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 52.0
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
SMAD2 (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90A549heart
Method: immunoblot
Attachment from submitter
not compliant
Caption
a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, Hela S3, HepG2, IMR90, A549, Heart using the antibody 600-401-A59. Molecular mass: 52306 Da
Reviewer comment
Bands not within 20% of expected size.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
SMAD2 (Homo sapiens)
HeLa
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: Hela, using the antibody 600-401-A59 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size 52 kDa.
Reviewer comment
IgG control shares same band
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
SMAD2 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 whole cell lysates using 600-401-A59, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was silver-stained, gel fragments corresponding to the bands indicated were excised and destained using the SilverSNAP Stain for Mass Spectrometry (Pierce). Then proteins were treated with trypsin using the in-gel digestion method. Digested proteins were analyzed on a LTQ-Orbitrap (Thermo Scientific) b the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (protein false discovery rate <1%, 2 peptides per protein minimum).
Reviewer comment
The detected peptide table seems quite short...
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download
SMAD2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation of Smad2 from K562 cells using 600-401-A59. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with 600-401-A59. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. Expected band size ~52kD.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download