1. Antibodies
  2. Homo sapiens
  3. WDR5

ENCAB975FNJ

Antibody against Homo sapiens WDR5

Homo sapiens
K562, HepG2, GM12878, endothelial cell of umbilical vein
not characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A302-429A
Lot ID
2
Characterized targets
WDR5 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Aliases
bradley-bernstein:PchAb 1270
External resources

Characterizations

WDR5 (Homo sapiens)
K562HepG2GM12878GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
2g nuclear lystate for each IP. 2ug of primary antibody and 50uL of pA/G beads. Used a 4-12% Bis-Tris gel in 1X MOPS running buffer. iBlot transfer, Program 3, 6 minutes. Blocked overnight at 4'C in TBS-T with 5% BSA. Antibodies in TBS-T with 5% BSA. Primary antibody overnight at 4'C. Secondary antibody used for 1 hour at RT. 0.75 second exposure in ChemiDoc. Expected band size at 36kDa.
Reviewer comment
KCO: expected band isn't >50% of all bands in its given lane.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
WDR5 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
Attachment from submitters
not compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (10 ug), GM12878 (10ug), and HUVEC (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. A range of bands were detected outside of the expected size (36kDa).
Reviewer comment
KCO: expected band isn't >50% of all bands in its given lane.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad