ENCAB975FNJ

Antibody against Homo sapiens WDR5

Homo sapiens
endothelial cell of umbilical vein, GM12878, K562, HepG2
not characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A302-429A
Lot ID
2
Host
rabbit
Clonality
polyclonal
Aliases
bradley-bernstein:PchAb 1270
External resources

Characterizations

WDR5 (Homo sapiens)
K562HepG2GM12878
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
2g nuclear lystate for each IP. 2ug of primary antibody and 50uL of pA/G beads. Used a 4-12% Bis-Tris gel in 1X MOPS running buffer. iBlot transfer, Program 3, 6 minutes. Blocked overnight at 4'C in TBS-T with 5% BSA. Antibodies in TBS-T with 5% BSA. Primary antibody overnight at 4'C. Secondary antibody used for 1 hour at RT. 0.75 second exposure in ChemiDoc. Expected band size at 36kDa.
Reviewer comment
KCO: expected band isn't >50% of all bands in its given lane.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
WDR5 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
not compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (10 ug), GM12878 (10ug), and HUVEC (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. A range of bands were detected outside of the expected size (36kDa).
Reviewer comment
KCO: expected band isn't >50% of all bands in its given lane.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad