ENCAB975CWB
Antibody against Homo sapiens RBM39
Homo sapiens
K562
characterized to standards
Homo sapiens
HepG2
characterized to standards with exemption
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A300-291A
- Lot ID
- 1
- Characterized targets
- RBM39 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- External resources
Characterizations
RBM39 (Homo sapiens)
K562HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-WB analysis of K562 whole cell lysate using RBM39 specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal anti-Caper antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitter comment
- This antibody was given and exemption for K562. The data was clean and the pattern is reliable.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- Bethyl_A300-291A_1_RBM39.png
RBM39 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against RBM39 in K562 and HepG2 whole cell lysate using RBM39 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBM39. Lanes 5-8 follow the same pattern, but in HepG2. RBM39 protein appears as the green band, GAPDH serves as a control and appears in red.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- RBM39_Secondary_Western.png