ENCAB969VGQ
Antibody against Homo sapiens H3K4me1, Mus musculus H3K4me1
Mus musculus
any cell type or tissue
characterized to standards
Homo sapiens
any cell type or tissue
characterized to standards
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab8895
- Lot ID
- GR114297
- Characterized targets
- H3K4me1 (Homo sapiens), H3K4me1 (Mus musculus)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, mono methylated at K4
- Aliases
- bing-ren:5.1
- External resources
Characterizations
H3K4me1 (Homo sapiens)
compliant
- Caption
- Performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-668, http://www.millipore.com/catalogue/item/16-668#). The top panel contains histone H2A, H2B, and H4 peptides while the bottom panel has histone H3 peptides. The antibody recognized the H3K4me1 peptide 98.2% (noted with red #1) and the H3K79me1 peptide 1.8% (noted with red #2).
- Submitted by
- Cricket Sloan
- Lab
- Bing Ren, UCSD
- Grant
- U54HG006997
- Download
- bing-ren_5.10_dotblot.jpeg
H3K4me1 (Mus musculus)
compliant
- Caption
- Performed dot blot analysis using Millipore’s AbSurance™ Complete Core Histone Antibody Specificity Array (cat# 16-668, http://www.millipore.com/catalogue/item/16-668#). The top panel contains histone H2A, H2B, and H4 peptides while the bottom panel has histone H3 peptides. The antibody recognized the H3K4me1 peptide 98.2% (noted with red #1) and the H3K79me1 peptide 1.8% (noted with red #2).
- Submitted by
- Ah Young Lee
- Lab
- Bing Ren, UCSD
- Grant
- U54HG006997
- Download
- bing-ren_5.10_dotblot.jpeg
H3K4me1 (Mus musculus)
liverlungstomach
compliant
- Caption
- An immunoblot using whole cell extract of three tissue types in mouse and one human colorectal carcinoma cell line shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
- Submitted by
- Ah Young Lee
- Lab
- Bing Ren, UCSD
- Grant
- U54HG006997
- Download
- bing-ren_5.10_Western.jpg
H3K4me1 (Homo sapiens)
HCT116
compliant
- Caption
- An immunoblot using whole cell extract of three tissue types in mouse and one human colorectal carcinoma cell line shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
- Submitted by
- Ah Young Lee
- Lab
- Bing Ren, UCSD
- Grant
- U54HG006997
- Download
- bing-ren_5.10_Western.jpg