ENCAB967KEH
Antibody against Homo sapiens SIN3A
Homo sapiens
MCF-7, A549, K562
characterized to standards
- Status
- released
- Source (vendor)
- Novus
- Product ID
- NB600-1263
- Lot ID
- MH158243
- Characterized targets
- SIN3A (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- A synthetic peptide made to an N-terminal portion of the mouse Sin3A protein (between residues 1-50)
- Aliases
- michael-snyder:AS-1658
- External resources
Characterizations
SIN3A (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
- Caption
- This is a new lot of a previously characterized antibody (ENCAB000AKR). New primary characterizations were done in the same cell types (K562 and MCF-7) as in the old lot and also in A549. A new secondary characterization should not be needed if the primaries meet the standard and the existing secondary characterization (IP-mass spec) for the lot of this antibody should be applicable.
- Submitted by
- Esther Chan
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- ENCAB967KEH_secondary.png
SIN3A (Homo sapiens)
A549
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: A549 using the antibody NB600-1263. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 145.175.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- SIN3A_A549.jpg
SIN3A (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562 using the antibody NB600-1263. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 145.175.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- SIN3A_K562.jpg
SIN3A (Homo sapiens)
MCF-7
Method: immunoprecipitation
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7 using the antibody NB600-1263. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 145.175.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- SIN3A_MCF7.jpg