ENCAB967KEH

Antibody against Homo sapiens SIN3A

Homo sapiens
A549, MCF-7, K562
characterized to standards
Status
released
Source (vendor)
Novus
Product ID
NB600-1263
Lot ID
MH158243
Characterized targets
SIN3A (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
A synthetic peptide made to an N-terminal portion of the mouse Sin3A protein (between residues 1-50)
Aliases
michael-snyder:AS-1658
External resources

Characterizations

SIN3A (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment from submitter
compliant
Caption
This is a new lot of a previously characterized antibody (ENCAB000AKR). New primary characterizations were done in the same cell types (K562 and MCF-7) as in the old lot and also in A549. A new secondary characterization should not be needed if the primaries meet the standard and the existing secondary characterization (IP-mass spec) for the lot of this antibody should be applicable.
Submitted by
Esther Chan
Lab
Michael Snyder, Stanford
SIN3A (Homo sapiens)
A549
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: A549 using the antibody NB600-1263. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 145.175.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
SIN3A (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562 using the antibody NB600-1263. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 145.175.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
SIN3A (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7 using the antibody NB600-1263. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 145.175.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download