1. Antibodies
  2. Homo sapiens
  3. MIER1

ENCAB956LLD

Antibody against Homo sapiens MIER1

Homo sapiens
K562
characterized to standards with exemption
Status
released
Source (vendor)
Sigma
Product ID
HPA019589
Lot ID
R08028
Characterized targets
MIER1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Mesoderm induction early response protein 1 recombinant protein epitope signature tag (PrEST)
Antigen sequence
MMEGETNFSSEIEDLAREGDMPIHELLSLYGYGSTVRLPEEDEEEEEEEEEGEDDEDADNDDNSGCSGENKEENIKDSSGQEDETQSSNDDPSQSVASQDAQEI
Aliases
michael-snyder:748
External resources

Characterizations

MIER1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
exempt from standards
Caption
Immunoprecipitation of MIER1 from K562 cells using HPA019589. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with HPA019589. Lane 3: material immunoprecipitated using control IgG. Band A and BandB were excised from gel and subject to analysis by mass spectrometry. The expected band size is 58 kDa.
Submitter comment
Mass spec shows target in band A.
Reviewer comment
Multiple bands at higher than expected size, but target confirmed by mass-spec.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download
 1006_MIER1.jpg
MIER1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody HPA019589. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
too many bands, not >50% of total signal
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
MIER1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using HPA019589, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Reviewer comment
BAHD1 has same unique peptide count and is also DNA binding. Do they interact?
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford