ENCAB953TNR
Antibody against Homo sapiens DROSHA
Homo sapiens
K562, HepG2
characterized to standards
- Status
- released
- Source (vendor)
- Cell Signaling
- Product ID
- 3364
- Lot ID
- 3
- Characterized targets
- DROSHA (Homo sapiens)
- Host
- rabbit
- Clonality
- monoclonal
- Isotype
- IgG
- Antigen description
- A synthetic peptide corresponding to residues surrounding Gly953 of human Drosha
- Aliases
- xiang-dong-fu:DROSHA
- External resources
Characterizations
DROSHA (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following CRISPR against DROSHA in HepG2 whole cell lysate using DROSHA specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DROSHA. DROSHA protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- DROSHA-HEPG2-CRISPR.png
DROSHA (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using DROSHA specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '30% input') , lane 2 is 10% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 10% IP enrichment using rabbit monoclonal anti-DROSHA antibody (lanes under 'anti-DROSHA'). Asterisk indicates heavy chain of antibody.
- Submitted by
- Marcus Ho
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- DROSHA_HepG2.png
DROSHA (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of K562 whole cell lysate using DROSHA specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '30% input') , lane 2 is 10% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 10% IP enrichment using rabbit monoclonal anti-DROSHA antibody (lanes under 'anti-DROSHA'). Asterisk indicates heavy chain of antibody.
- Submitted by
- Marcus Ho
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- DROSHA_K562.png