ENCAB953TNR

Antibody against Homo sapiens DROSHA

Homo sapiens
K562, HepG2
characterized to standards
Status
released
Source (vendor)
Cell Signaling
Product ID
3364
Lot ID
3
Characterized targets
DROSHA (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Isotype
IgG
Antigen description
A synthetic peptide corresponding to residues surrounding Gly953 of human Drosha
Aliases
xiang-dong-fu:DROSHA
External resources

Characterizations

DROSHA (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
compliant
Caption
Western blot following CRISPR against DROSHA in HepG2 whole cell lysate using DROSHA specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DROSHA. DROSHA protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Submitted by
Xintao Wei
Lab
Brenton Graveley, UConn
DROSHA (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of HepG2 whole cell lysate using DROSHA specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '30% input') , lane 2 is 10% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 10% IP enrichment using rabbit monoclonal anti-DROSHA antibody (lanes under 'anti-DROSHA'). Asterisk indicates heavy chain of antibody.
Submitted by
Marcus Ho
Lab
Xiang-Dong Fu, UCSD
DROSHA (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
IP-Western Blot analysis of K562 whole cell lysate using DROSHA specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '30% input') , lane 2 is 10% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 10% IP enrichment using rabbit monoclonal anti-DROSHA antibody (lanes under 'anti-DROSHA'). Asterisk indicates heavy chain of antibody.
Submitted by
Marcus Ho
Lab
Xiang-Dong Fu, UCSD
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