ENCAB947YBH
Antibody against Homo sapiens ASH2L
Homo sapiens
HepG2, endothelial cell of umbilical vein, K562, HEK293, GM12878, A549, H1
characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A300-489A
- Lot ID
- 2
- Characterized targets
- ASH2L (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Antigen description
- ash2 (absent, small, or homeotic)-like (Drosophila)
- Aliases
- bradley-bernstein:PchAb 42-V
- External resources
Characterizations
ASH2L (Homo sapiens)
GM12878A549H1
compliant
- Caption
- Nuclear lysates from GM12878 (10ug), A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected at 50kDa, 60kDa and 80kDa. Expected bands at 65kDa and 80kDa.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- ASH2_Bethyl_A300-489A-2-V2.png
ASH2L (Homo sapiens)
HepG2endothelial cell of umbilical vein
compliant
- Caption
- Nuclear lysates from HUVEC (10ug), HepG2 (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MESrunning buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. two bands at expected sizes can be detected (68kDa and 80kDa).
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
ASH2L (Homo sapiens)
compliant
- Caption
- This validation relies on the use of antibodies to different members of a known complex, in GM12878 cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The first track shown used an antibody to ASH2L (PchAb 42-V), and the second track shown used an antibody to RbBP5 (PchAb 153-V), another member of the histone methyltransferase complex. Overall correlation score: 0.8600
- Submitter comment
- SAV1
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- ASH2L PchAb 42-V SAV.pdf
ASH2L (Homo sapiens)
K562HEK293
compliant
- Caption
- 1.5X10e6 cells equivalent of nuclear extract from HEK293 and K562 cells, were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a single band of the expected size was detected (~68kDa).
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991