ENCAB947YBH

Antibody against Homo sapiens ASH2L

Homo sapiens
HepG2, endothelial cell of umbilical vein, K562, HEK293, GM12878, A549, H1
characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A300-489A
Lot ID
2
Characterized targets
ASH2L (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Antigen description
ash2 (absent, small, or homeotic)-like (Drosophila)
Aliases
bradley-bernstein:PchAb 42-V
External resources

Characterizations

ASH2L (Homo sapiens)
GM12878A549H1
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from GM12878 (10ug), A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected at 50kDa, 60kDa and 80kDa. Expected bands at 65kDa and 80kDa.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
ASH2L (Homo sapiens)
HepG2endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from HUVEC (10ug), HepG2 (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MESrunning buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. two bands at expected sizes can be detected (68kDa and 80kDa).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
ASH2L (Homo sapiens)
Method: ChIP-seq comparison
compliant
Caption
This validation relies on the use of antibodies to different members of a known complex, in GM12878 cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The first track shown used an antibody to ASH2L (PchAb 42-V), and the second track shown used an antibody to RbBP5 (PchAb 153-V), another member of the histone methyltransferase complex. Overall correlation score: 0.8600
Submitter comment
SAV1
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
ASH2L (Homo sapiens)
K562HEK293
Method: immunoblot
Attachment from submitter
compliant
Caption
1.5X10e6 cells equivalent of nuclear extract from HEK293 and K562 cells, were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a single band of the expected size was detected (~68kDa).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad