ENCAB946VDH
Antibody against Homo sapiens DDX5
Homo sapiens
HepG2
characterized to standards
- Status
- released
- Source (vendor)
- Cell Signaling
- Product ID
- 9877
- Lot ID
- 1
- Characterized targets
- DDX5 (Homo sapiens)
- Host
- rabbit
- Clonality
- monoclonal
- Isotype
- IgG
- Antigen description
- A synthetic peptide corresponding to residues surrounding Ser498 of human DDX5 protein
- Aliases
- xiang-dong-fu:DDX5
- External resources
Characterizations
DDX5 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against DDX5 in HepG2 whole cell lysate using DDX5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX5. DDX5 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- DDX5-HEPG2-FU'S.png
DDX5 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- IP-Western Blot analysis of HepG2 whole cell lysate using DDX5 specific antibody. Lane 1 is 1% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit monoclonal anti-DROSHA antibody (lanes under 'anti-DDX5'). Asterisk indicates heavy chain of antibody.
- Submitted by
- Jean Davidson
- Lab
- Xiang-Dong Fu, UCSD
- Grant
- U54HG007005
- Download
- DDX5_HepG2.png